Ramón Latorre

Estructura y Función de Sensores Molecularesramon-latorre

Director del Centro Interdisciplinario de Neurociencia de Valparaíso 
Profesor Titular del Instituto de Neurociencia de la Universidad de Valparaíso 
Académico Asociado, University of Chicago – Profesor Adjunto, University of California, Los Angeles, USA.
Miembro del Consejo de la Academia de Ciencias de América Latina y Miembro Extranjero de la Academia Nacional de Ciencias, USA.

Contact information:

E-mail: ramon.latorre at uv.cl
Teléfono: (56)-(32)-2508040
Fax: (56)-(32)-2508027
Dirección: Centro Interdisciplinario de Neurociencia de Valparaíso.
Facultad de Ciencias, Universidad de Valparaiso.
Pasaje Harrington 287, Playa Ancha. Valparaíso. Chile.

Curriculum Vitae

Research Statement:

Ion channels are membrane proteins activated by sensing stimuli such as membrane voltage, ligand binding or temperature and transduce this information into conformational changes that open the channel pore. A key question that my laboratory is trying to answer is how do the protein domains involved in sensing stimuli (sensors) and opening the pore (gates) communicate. We are interested in two type of channels transient receptor potential (TRP) channels that confer thermosensation and Ca2+- and voltage-activate K+ channels because they are unique to the extent that they integrate a variety of physical and chemical stimuli.

 

 Selected Publications:

  • Castillo, JP, Rui, H, Basilio, D, Das, A, Roux, B, Latorre R, Bezanilla, F, Holmgren, M. (2015). Mechanism of potassium ion uptake by the Na(+)/K(+)-ATPase. Nat Commun. 6:7622
  • Castillo K, Contreras GF, Pupo A, Torres Y, Neely A, Gonzalez C and Latorre R. (2015) Molecular mechanism underlying β1 regulation in voltage- and calcium-activated potassium (BK) channels. Proc Natl Acad Sci U S A. 112(15):4809-14
  • Jabba S, Goyal R, Sosa-Pagán JO, Moldenhauer H, Wu J, Kalmeta B, Bandell M, Latorre R, Patapoutian A, Grandl J. (2014) Directionality of Temperature Activation in Mouse TRPA1 Ion Channel Can Be Inverted by Single-Point Mutations in Ankyrin Repeat Six. Neuron. 82(5):1017-1031
  • Cea LA, Cisterna BA, Puebla C, Frank M, Figueroa XF, Cardozo C, Willecke K, Latorre R, Sáez JC. (2013) De novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy. Proc Natl Acad Sci U S A. 110(40):16229-34.
  • Contreras GF, Neely A, Alvarez O, Gonzalez C, Latorre R. (2012) Modulation of BK channel voltage gating by different auxiliary β subunits. Proceedings of the National Academy of Sciences. 109(46):18991-18996.
  • Latorre, R, Brauchi, S., Madrid, R. and Orio, P. (2011). A Cool Channel in Cold Transduction. Physiolgy 26: 273-285.
  • Castillo, J.P. De Giorgis, D., Basilio, D., Gadsby, D.C., Rosenthal, J.J., Latorre, R., Holmgren, M., and Bezanilla, F. (2011). Energy landscape of the reaction governing the Na+ deeply occluded state of the Na+/K+ -ATPaser in the giant axon of the Humboldt squid. Proc. Natl. Acad. Sci. (USA). 108:20556-61
  • Latorre, R., Zaelzer, C., and Brauchi, S.(2009). Structure and functional intimacies of transient receptor potential (TRP) channels. Quart. Rev. Biophys. 42:201-246
  • Latorre R. (2009) Perspectives on TRP Channel Structure and the TRPA1 Puzzle. J Gen Physiol. Mar;133(3):227-9
  • Carvacho, I., Gonzalez, W., Torres, Y., Brauchi, S., Alvarez, O., Gonzalez-Nilo, F.D., and  Latorre  R. (2008).  Intrinsic Electrostatic Potential in the BK Channel Pore: Role in Determining Single Channel Conductance and Block.  Journal of General Physiology. 31:147-162
  • Brauchi S., Orta G., Mascayano C., Salazar M., Raddatz N., Urbina H.,Rosenman E., Gonzalez-Nilo F., Latorre R. (2007). Dissection of the components for PIP2 activation and thermosensation in trp channels. Proc. Natl. Acad. Sci. (USA) 104:10246-10251.
  • Latorre, R., Brauchi, S., Orta, G., Zaelzer, C., Vargas, G.  (2007). ThermoTRP Channels as Modular Proteins with Allosteric Gating. Cell Calcium 42:427-438.
  • Morera, F., Vargas, G., and Latorre, R.  2007. Ion Channel Reconstitution. In: Protocols in Biophysics to Study membrane Lipids. Dopico, A. Ed. Humana Press, NJ. 400:571-85
  • Torres Y., Morera, FJ., Carvacho, I., and Latorre, R. (2007).  A marriage of convenience:  beta-subunits of voltage-dependent channels.  J. Biol. Chem. 282:24495-24489. Review
  • Orio, P., Torres Y., Rojas, P., Carvacho, I., Garcia, ML., Toro, L., Valverde, M. And Latorre, R. (2006). Structural determinants for functional coupling between the beta and alpha subunits in the Ca2+-activated K+ (BK) channel. J. Gen. Physiol. 127:191-204.