Satellite Workshop
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Those interested in attending this workshop should send email to:
agustin.martinez@uv.cl
jcsaezc@gmail.com
Biophysic of hemichannels and gap junction channels: A theoretical and practical training.
Places:
Pontificia Universidad Católica de Chile, Santiago.
Centro Interdisciplinario de Valparaíso, Valparaíso.
Organizers:
Dr. Agustín D. Martínez (CINV, Univ. Valparaíso, Chile),
Dr. Mauricio Retamal (UDD, Chile)
Dr. Juan C. Sáez (CINV and PUCC, Chile)
Funded by:
International Brain Research Organization (IBRO)
Universidad de Valparaíso (UV)
Centro Interdisciplinario de Neurociencias de Valparaíso (CINV)
Pontificia Universidad Católica de Chile (PUCC)
Universidad del Desarrollo (UDD)
Anillo ACT-1104
Tuesday, March 24 (PUCC, Santiago)
14:00-14:30 Registration.
Topic 1.- Advances in channel properties using electrophysiology and fluorescent approaches
14:30-15:15 “What you would like to know about ion channel and you are afraid to ask”.
Ramón Latorre. Centro Interdisciplinario de Neurociencia de Valparaíso. Universidad de Valparaíso. Chile.
15:15-16:00 “Use of Fluorescent Probes to Further Understand Membrane channels: the new connexin era”.
Carlos González. Centro Interdisciplinario de Neurociencia de Valparaíso. Universidad de Valparaíso. Chile.
16:00-16:15 Coffee break
Topic 2: Experimental Approaches to Study Gating and Permeability of Connexin-Based Channels (PUCC, Santiago)
16:15 – 17:00 “Structure function study of gap junction docking, gating and unitary channel conductance”.
Donglin Bai, University of Western Ontario, Canada.
17:00 – 17:45 “Voltage-dependent Gating in Cx Hemichannels”.
Vytautas Verselis. Albert Einstein College of Medicine, USA.
17:45-18:30 “Hemichannel currents: more than ups and downs”.
Jorge E. Contreras, Rutgers University, USA.
19:30 Welcome dinner.
Wednesday, March 25 (PUCC, Santiago)
Topic 2: Experimental Approaches to Study Gating and Permeability of Connexin-Based Channels (PUCC, Santiago)
9:00-09:45 “Unitary activities of connexin hemichannels: recordings in cardiomyocytes and analysis”..
Nan Wang, Ghent University, Belgium.
9:45-10:30 “Planar lipid bilayers and ion channels reconstitution to study biophysical properties of hemichannels”.
Mohamed Kreir. Application Scientist, Research. Nan]i[on Technologies GmbH. Munich. Germany
10:30-10:45 Coffee break
10:45-11:30 “Purification and reconstitution of connexin hemichannels and its functional evaluation in liposomes”..
Guillermo Altenberg, Texas Tech UniversityTexas Tech University, USA.
11:30-12:15 “Permeability properties of hemichannels in real time”..
Juan C. Sáez. Pontificia Universidad Católica de Chile, Chile.
12:15-13:00 “Regulation of hemichannels by unsaturated fatty acids and gases..
Mauricio Retamal. Universidad del Desarrollo, Chile.
13:00-15:00 Lunch
Topic 3. Gap Junction Channels and Hemichannels: properties and Regulatory Mechanisms.
15:00-15:45 “Molecular and functional asymmetry at a vertebrate electrical synapse”..
Alberto Pereda, Albert. Einstein College of Medicine, USA
15:45-16:30 “Investigating Pannexin-1 during Synaptic Plasticity”. .
Roger Thompson, University of Calgary, Canada.
16:30-16:45 Coffee break
16:45-17:30 “Imaging of connexins and pannexins: Regulation by cytoskeleton”. .
Agustín Martínez. CINV, University of Valparaíso, Chile.
19:30- Dinner
Thursday, March 26 (PUCC, Santiago)
Practical demonstrations
9:00 Real time Recording of HC activity by fluorescent dye uptake technique.
Bruno Cisterna, Paola Fernandez. PUC C.
Recording of HC-current by whole cell patch clamp.
Nan Wang and Anibal Vargas. PUCC.
9:00 Recording HC-currents in Xenopus oocuytes (Whole cell voltage-clamp) and in transfected cells (Port-a-Patch). .
Mauricio Retamal. UDD.
18:00 Trip to Valparaiso to join the IGJC 2015
21:00 Dinner in Valparaíso
Friday, March 27 (Centro Interdisciplinario de Neurociencia de Valparaíso)
9:00- Recording of HC activity by two electrode voltage clamp in Xenopus oocyte (TEVC).
Jorge Contreras, William López, Isaac García, Bernardo Pinto.
Recording of GJ activity by dual whole voltage clamp.
Alex Vielma, Oscar Jara.
13:00- Lunch
14:00- Recording of HC-current by whole cell patch clamp using Port-a-Patch.
Mohamed Kreir, Bernardo Pinto, Isaac García.
14:00- Recording of GJ activity by fluorescent techniques (dye coupling and FRAP).
Jaime Maripillán, Agustín Martínez.
Information to contact the speakers
Ramón Latorre – ramon.latorre@uv.cl
Carlos González – carlos.gonzalez@cinv.cl
Donglin Bai – donglin.bai@schulich.uwo.ca
Vytautas Verselis – vytas.verselis@einstein.yu.edu
Jorge E. Contreras – contrejo@njms.rutgers.edu
Nan Wang – antiquewn@gmail.com
Mohammed Kreir- mohamed.kreir@nanion.de
Guillermo Altenberg – g.altenberg@ttuhsc.edu
Alberto Pereda – alberto.pereda@einstein.yu.edu
Roger Thompson – neurophy@yahoo.com
Agustín D. Martínez – agustin.martinez@uv.cl
Juan C. Sáez- jsaez@bio.puc.cl
Mauricio Retamal – mretamal@udd.cl
William López – student7@live.com
Anibal Vargas – jugomaster@gmail.com
Isaac Garcia – atekphd@gmail.com
Bernardo Pinto – trowton@gmail.com
Alex Vielma – alexvielma@gmail.com
Oscar Jara – jara.oscar@gmail.com
Jaime Maripillán – jaime.maripillan@gmail.com