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Line 2: Cellular signaling.

2- This line studies the molecular mechanisms that allow cell signaling, an essential process for the adequate communication between cells and that results crucial for the correct functioning of the system within the optimal parameters of signal transmission, both in time and in intensity. In this context, this group focuses on studying the molecular structures of certain connexins and their mutations, which can produce syndromial-non syndromical deafness as well as inflammatory responses in neurodegenerative illnesses and in synaptic plasticity processes, neuronal excitability, neurosecretion and skeletal muscle contraction potentiation. Together with connexins this group also studies the role of dinamin-2, an enzyme responsible for endocytosis (mechanism that allows the selective entry of molecules into the cell) y whose mutations can alter the intracellular traffic of proteins that are required for the skeletal muscle metabolism, leading to the development of myopathies, diseases of the muscular tissue that are of great incidence in Chile. Techniques used by this group include molecular, electrophysiological, fluorescence and molecular dynamics, using both cell culture and mice and model systems.

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Researchers.

Juan Carlos Sáez (Director Alterno CINV) / fono:(56) 2 3542862 / jsaez at bio.puc.cl
Ana María Cárdenas / fono:(56) 32 2508052 / ana.cardenas at uv.cl
Agustín Martínez / fono:(56) 32 2508415 / agustin.martinez at uv.cl

Associate Researchers.

Postdoctoral Researchers.

Adam Aguirre / fono (56) 2 6862860 / aaducler at yahoo.es
Arlek González / fono:(56) 32 2508022 / arlek.gonzalez at cinv.cl
Carlos Puebla / fono: (56) 2 3542860 / carlospuebla81 at gmail.com
Pavel Prado / fono:(56) 32 2508023 / pavel.prado at cinv.cl
Rosalba Escamilla / fono:(56) 2 3542860 / rescamhdz at yahoo.com
María Constanza Maldifassi /  fono:(56) 32 2508022 /  constanza.maldifassi at cinv.cl

PhD Students.

Anibal Vargas / fono: (56) 2 3542860 / jugomaster at gmail.com
Bruno Cistrerna / fono: (56) 2 3542860 / br1cisterna at gmail.com
Carola Maturana / fono:(56) 32 3542860 / carola.maturana at cinv.cl
Daniela Salas / fono: (56) 2 3542860 / paharcha at gmail.com
Dusan Racordón/ fono: (56)2 3542860 / racord at gmail.com
Elsa Fritz / fono: (56)2 3542860 / mielsita@gmail.com
Fernando Hinostroza / fono:(56) 32 2508022 / fernando.hinostroza at cinv.cl
Gonzalo Gómez: / fono: (56) 2 3542860 / gggonzag at gmail.com
Paloma Harcha / fono: (56) 2 3542860 / paharcha at gmail.com
Ana Abbott / fono:(56) 32 2508103 / ana.abbott at cinv.cl

  • García IE, Sánchez HA, Martinez AD, Retamal MA. (2018) Redox-mediated regulation of connexin proteins; focus on nitric oxide. Biochim Biophys Acta. 1860(1):91-95 doi: 10.1016/j.bbamem.2017.10.006. Review
  • Cárdenas AM, Fernández-Olivares P, Díaz-Franulic I, González-Jamett AM, Shimahara T, Segura-Aguilar J, Caviedes R, Caviedes P. (2017) Knockdown of Myo-Inositol Transporter SMIT1 Normalizes Cholinergic and Glutamatergic Function in an Immortalized Cell Line Established from the Cerebral Cortex of a Trisomy 16 Fetal Mouse, an Animal Model
  • Charvériat M, Naus CC, Leybaert L, Sáez JC, Giaume C. (2017) Connexin-Dependent Neuroglial Networking as a New Therapeutic Target. Front Cell Neurosci. 2017 Jun 26;11:174. doi: 10.3389/fncel.2017.00174. eCollection 2017. Review.
  • Balboa E, Saavedra F, Cea LA, Vargas AA, Ramírez V, Escamilla R, Sáez JC, Regueira T. (2017). Sepsis-Induced Channelopathy in Skeletal Muscles is Associated with Expression of Non-Selective Channels. Shock. 2017 May 30. doi: 10.1097/SHK.0000000000000916. [Epub ahead of print]
  • Cea LC, Cisterna BA, Puebla C, Frank M, Figueroa XF, Cardozo C, Willecke K, Latorre R, Sáez JC (2013). De novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy. Proc Natl Acad Sci USA 110(40):16229-16234.
  • Schalper KA, Palacios-Prado N, Retamal MA, Shoji KF, Martínez AD, Sáez JC (2008). The connexin hemichannel composition determines the FGF-1-induced membrane permeability and free Ca2+i responses. Mol Biol Cell 19(8): 3501–3513.
  • González-Jamett AM, Momboisse F, Haro-Acuña V, Bevilacqua JA, Caviedes P, Cárdenas AM (2013). Dynamin-2 function and dysfunction along the secretory pathway. Front Endocrinol 4:126.
  • Jara O, Acuña R, García IE, Maripillán J, Figueroa V, Sáez JC, Araya-Secchi R, Lagos CF, Perez-Acle T, Berthoud VM, Beyer EC, Martínez AD (2012). Critical role of the first transmembrane domain of Cx26 in regulating oligomerization and function. Mol Biol Cell 23(17):3299-3311.
  • González-Jamett AM, Momboisse F, Guerra MJ, Ory S, Báez-Matus X, Barraza N, Calco V, Houy S, Couve E, Neely A, Martínez AD, Gasman S, Cárdenas AM (2013). Dynamin-2 regulates fusion pore expansion and quantal release through a mechanism that involves actin dynamics in neuroendocrine chromaffin cells. PLoS One 8(8):70638.
  • García IE, Maripillán J, Jara O, Ceriani R, Palacios-Muñoz A, Ramachandran J, Olivero P, Perez-Acle T, González C, Sáez JC, Contreras JE, Martínez AD (2015). Keratitisichthyosis-deafness syndrome-associated Cx26 mutants produce nonfunctional gap junctions but hyperactive hemichannels when co-expressed with wild type Cx43. J Invest Dermatol 135(5): 1338-47.

Ongoing projects.

Instituto Milenio-CINV

Año: 2016-2021
Concurso: Proyectos Institutos Milenio 2009 – ICM09-022-P
Financiamiento:  Ministerio de Economía, Fomento y Turismo de Chile
Investigador Principal: Ramón Latorre
Otros Investigadores: Juan Carlos Sáez, Alan Neely, Ana María Cárdenas, John Ewer, Kathleen Whitlock, Adrián Palacios, Tomás Pérez-Acle, David Naranjo, Fernando González-Nilo, Oliver Schmachtenberg, Agustín Martínez, Patricio Orio, Andrés Chávez, Pablo Moya, Andrea Calixto, Carlos González.
Otras Universidades o Instituciones: Centro Interdisciplinario de Neurociencia de Valparaíso, Pontificia Universidad Católica, Universidad Andrés Bello, Fundación Ciencia y Vida.

CONICYT

MOLECULAR MECHANISMS OF CONNEXIN HEMICHANNEL HYPERACTIVITY IN KID (KERATITIS-ICHTHYOSIS-DEAFNESS) SYNDROME
Año: abril 2017 – abril 2020
Concurso: Fondecyt Regular 2017 – # 1171240
Financiamiento: Conicyt
Investigador Principal: Agustín Martínez
Otros Investigadores: John Ewer

IS THERE A VOLTAGE-CONTROLLED HYDRAULIC GATING IN THE HUMAN CONNEXIN 26 HEMICHANNEL?

Años: marzo 2016 – marzo  2020
Concurso: Fondecyt Regular 2016 – # 1160574
Financiamiento: Conicyt
Investigador Principal: Tomás Pérez-Acle
Otros Investigadores: Agustín Martínez

ROLE OF DYSFERLIN IN CYTOSKELETAL ACTIN REMODELING AND ITS IMPACT ON VESICLE TRAFFICKING AND MEMBRANE REPAIR IN MUSCULAR DYSTROPHY LINKED TO DYSFERLIN MUTATIONS

Año: 1 abril 2016 – 1 abril  2020
Concurso: Fondecyt Regular 2016 – # 1160495
Financiamiento: Conicyt
Investigador Principal: Ana María Cárdenas
Otros Investigadores: Pablo Caviedes
Otras Universidades o Instituciones: Universidad de Chile

NEURONAL DERIVED FACTOR(S) AND MECHANISM(S) THAT REPRESS THE EXPRESSION OF NONSELECTIVE ION CHANNELS IN NORMAL ADULT SKELETAL MUSCLES

Año: marzo 2015 – marzo 2019
Concurso: Fondecyt Regular 2015 – #° 1150291
Financiamiento: Conicyt
Investigador Principal: Juan Carlos Sáez
Coinvestigador(es): Bruno Cisterna, Rosalba Escamilla, Carlos Puebla
Instituciones coinvestigador(es): Pontificia Universidad Católica de Chile

CONNEXIN HEMICHANNELS AS MEDIATORS OF SARCOLEMMAL AND MITOCHONDRIAL DYSFUNCTION IN RESPIRATORY AND PERIPHERAL MUSCLE IN THE PATHOGENESIS OF SEPSIS ASSOCIATED-INTENSIVE CARE UNIT ACQUIRED WEAKNESS (ICUAW)

Año: marzo 2014 – marzo 2018
Concurso: Fondecyt Regular 2014 – # 1141092
Financiamiento: Conicyt
Investigador Principal: Tomas Regueira Heskia
Otros Investigadores: Juan Carlos Sáez, Anibal Vargas, Luis Cea
Otras Universidades o Instituciones: Pontificia Universidad Católica de Chile

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