Microscopy Services – Centro de Neurociencia Valparaíso

Microscopy Services

The CINV approaches different aspects of a fundamental scientific question; how does the NS respond to the different sensory stimuli in the healthy and the diseased brain? This question is approached through the different research lines resent in the center, studying from the functioning of proteins that capture signals from the outside world up to the behavior of different neural networks.

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CONFOCAL

Confocal Microscopy Laboratory

The advanced fluorescence microscopy laboratory has a laser Confocal Microscope to take high resolution images and time lapse videos in three dimensions. Read more…

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document	date	13565486830000
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document	unit	au	au	au	au
document	document	average	0	0	0
document	document	profile	1
history	Acquisition	Acquire.Scanner.Name	Confocal C1 Scanner
history	Acquisition	Acquire.Scanner.XField.DisplayString	199.2  um
history	Acquisition	Acquire.Scanner.YField.DisplayString	199.2  um
history	Acquisition	Acquire.Scanner.XPosition.DisplayString	-12.1  um
history	Acquisition	Acquire.Scanner.YPosition.DisplayString	-17.8  um
history	Acquisition	Acquire.Scanner.Angle	-15
history	Acquisition	Acquire.Scanner.ScanMode	0
history	Acquisition	Acquire.Scanner.TransmittedDetector	0
history	firmware	4.11
history	Acquisition	Acquire.Scanner.ClemMode	0
history	Acquisition	Modules("Confocal C1 Grabber").BarrierFilter(1)	BA 450/35
history	Acquisition	Modules("Confocal C1 Grabber").BarrierFilter(2)	BA 605/75
history	Acquisition	Modules("Confocal C1 Grabber").BarrierFilter(3)	BA 515/30
history	Acquisition	Modules("Confocal C1 Grabber").DichroicMirror(1)	DM 480
history	Acquisition	Modules("Confocal C1 Grabber").DichroicMirror(2)	DM 545
history	Acquisition	Modules("Confocal C1 Grabber").EnableChannel(1)	False
history	Acquisition	Modules("Confocal C1 Grabber").EnableChannel(2)	False
history	Acquisition	Modules("Confocal C1 Grabber").EnableChannel(3)	True
history	Acquisition	Modules("Confocal C1 Grabber").EnableChannel(4)	False
history	Acquisition	Modules("Confocal C1 Grabber").LinearGainMode	True
history	Acquisition	Modules("Confocal C1 Grabber").PixelTimeCorrection	True
history	Acquisition	Modules("Confocal C1 Grabber").Offset(1)	100
history	Acquisition	Modules("Confocal C1 Grabber").Offset(2)	105
history	Acquisition	Modules("Confocal C1 Grabber").Offset(3)	95
history	Acquisition	Modules("Confocal C1 Grabber").Offset(4)	127
history	Acquisition	LaserBox.MountedLasers.Count	3
history	Acquisition	LaserBox.Name	Confocal C1 LaserBox
history	Acquisition	LaserBox.Lasers(1).IntensityControlChannel	4
history	Acquisition	LaserBox.Lasers(2).IntensityControlChannel	1
history	Acquisition	LaserBox.Lasers(3).IntensityControlChannel	2
history	Acquisition	LaserBox.Lasers(4).IntensityControlChannel	0
history	Acquisition	LaserBox.Lasers(5).IntensityControlChannel	0
history	Acquisition	LaserBox.Lasers(6).IntensityControlChannel	0
history	Acquisition	LaserBox.Lasers(7).IntensityControlChannel	0
history	Acquisition	Acquire.ZStack.Top.DisplayString	8.00  um
history	Acquisition	Acquire.ZStack.Reference.DisplayString	0.00  um
history	Acquisition	Acquire.ZStack.Bottom.DisplayString	-2.00  um
history	Acquisition	Acquire.ZStack.Range.DisplayString	11.00  um
history	Acquisition	Acquire.ZStack.StepSize.DisplayString	1.00  um
history	laser	power	not measured
history	PassCount	3
history	objective	Plan Fluor 40.0x/1.30/0.20 Oil spring-loaded, stopper
history	objective	Type	Plan Fluor
history	objective	Magnification	40.0
history	objective	NA	1.300
history	objective	WorkingDistance	0.20
history	objective	Immersion	Oil
history	detector	standard
history	gain1	0.00
history	gain2	7.35
history	gain3	0.00
history	gain4	0
history	dwell	5.52 us
history	linetime	8064.72 us
history	frametime	8466.99 ms
history	shutter1	closed
history	shutter2	closed
history	shutter3	automatic
history	shutter4	closed
history	shutter5	closed
history	shutter6	closed
history	shutter7	closed
history	pinhole	33.3 um
history	date	Monday, October 22, 2012 12:45:58 (-3:00)
history	dateGMT	Monday, October 22, 2012 15:45:58
history	laser4	wavelength	477 nm
history	laser4	name	476
history	laser4	intensity	Min
history	laser5	wavelength	488 nm
history	laser5	name	488
history	laser5	intensity	50.0 %
history	laser6	wavelength	515 nm
history	laser6	name	514
history	laser6	intensity	Min
history	laser7	wavelength	561 nm
history	laser7	name	561
history	laser7	intensity	Min
history	software	EZ-C1
history	version	Gold Version 3.90 build 869
history	laser1	wavelength	408 nm
history	laser1	name	408
history	laser1	intensity	14 %
history	laser2	wavelength	488 nm
history	laser2	name	488
history	laser2	intensity	16.0 %
history	laser3	wavelength	544 nm
history	laser3	name	543
history	laser3	intensity	16.0 %
history	Acquisition	Acquire.LineChannelSeries.Enable	False
history	Acquisition	Acquire.LineChannelSeries.PassCount	0
history	Acquisition	Acquire.TimeSeries.Enable	False
history	Acquisition	Acquire.TimeSeries.Speed	0
history	Acquisition	Acquire.TimeSeries.Delay	15.78
history	Acquisition	Acquire.TimeSeries.DelayUnit	2
history	Acquisition	Acquire.TimeSeries.Number	20
history	Acquisition	Acquire.TimeSeries.NumberOfGroups	3
history	Acquisition	Acquire.TimeSeries.NumberAt(1)	10
history	Acquisition	Acquire.TimeSeries.NumberAt(2)	10
history	Acquisition	Acquire.TimeSeries.NumberAt(3)	10
history	Acquisition	Acquire.TimeSeries.DelayAt(1)	5
history	Acquisition	Acquire.TimeSeries.DelayAt(2)	10
history	Acquisition	Acquire.TimeSeries.DelayAt(3)	20
history	Acquisition	Acquire.TimeSeries.DelayUnitAt(1)	2
history	Acquisition	Acquire.TimeSeries.DelayUnitAt(2)	2
history	Acquisition	Acquire.TimeSeries.DelayUnitAt(3)	2
history	Acquisition	Acquire.TimeSeries.BleachAt(1)	0
history	Acquisition	Acquire.TimeSeries.BleachAt(2)	0
history	Acquisition	Acquire.TimeSeries.BleachAt(3)	0
history	Acquisition	Acquire.TimeSeries.SaveToFile	0
history	Acquisition	Acquire.LineMode	False
history	Acquisition	Acquire.ZStack.Enable	True
history	Acquisition	objective position	3
history	Acquisition	Acquire.ChannelSeries.Enable	True
history	Acquisition	Acquire.Average.Enable	False
history	Acquisition	Acquire.Average.StopAtCount	True
history	Acquisition	Acquire.Average.StopAtCountLimit	4
history	Acquisition	Acquire.Average.StopAtQuality	True
history	Acquisition	Acquire.Average.StopAtQualityLimit	12
history	objective	Detail	spring-loaded, stopper
history	step1	Name	Pass 1
history	step1	Pinhole	30 um small
history	step1	Channel 1	off
history	step1	Channel 2	off
history	step1	Channel 3	on
history	step1	Channel 4	off
history	step1	Gain 1	0
history	step1	Gain 2	110
history	step1	Gain 3	0
history	step1	Gain 4	0
history	step1	Shutter 1	closed
history	step1	Shutter 2	closed
history	step1	Shutter 3	automatic
history	step2	Name	Pass 2
history	step2	Pinhole	30 um small
history	step2	Channel 1	off
history	step2	Channel 2	on
history	step2	Channel 3	off
history	step2	Channel 4	off
history	step2	Gain 1	0
history	step2	Gain 2	0
history	step2	Gain 3	113
history	step2	Gain 4	0
history	step2	Shutter 1	closed
history	step2	Shutter 2	automatic
history	step2	Shutter 3	closed
history	step3	Name	Pass 3
history	step3	Pinhole	30 um small
history	step3	Channel 1	on
history	step3	Channel 2	off
history	step3	Channel 3	off
history	step3	Channel 4	off
history	step3	Gain 1	106
history	step3	Gain 2	0
history	step3	Gain 3	0
history	step3	Gain 4	0
history	step3	Shutter 1	automatic
history	step3	Shutter 2	closed
history	step3	Shutter 3	closed
sensor	type	IncohConfMicr
sensor	model	Nikon C1 Standard Detector
sensor	s_params	Channels	4
sensor	s_params	PinholeRadius	833	833	833
sensor	s_params	LambdaEx	543.5	543.5	543.5
sensor	s_params	LambdaEm	450	515	605
sensor	s_params	ExPhotonCnt	1	1	1
sensor	s_params	RefrInxMedium	1.515
sensor	s_params	RefrInxLensMedium	1.515
sensor	s_params	NumAperture	1.3
view1	Position	0.111458	0.006876
view1	Size	0.507813	0.859528
view1	Port	2
view1	Cursor	0.666667	0.079687	0.544531
view1	Color	2	0	2	1	0
view1	BlackLevel	0.000000	0.000000	0.000000
view1	Saturation	1.000000	1.000000	1.000000
view1	Gamma	1.000000	1.000000	1.000000
view1	IntZoom	0
view1	Live	1
view1	Synchronize	1
view1	ShowIndex	1
view1	AutoResize	0
view1	UseUnits	1
view1	Zoom	1.000000
view1	IgnoreAspect	0
view1	ShowCursor	0
view1	ShowAll	0
view1	Axis	0	0
view1	Order	0	1	2	3	4	5
view1	Tile	-1	-1
view1	scale	0.000000	255.000000	0.000000	0.000000	1.000000	0.000000	255.000000	0.000000	0.000000	1.000000	0.000000	255.000000	0.000000	0.000000	1.000000	0.000000	255.000000	0.000000	0.000000	1.000000
view1	DimViewOption	1	2	1	1	1	1
view1	Alpha	0.500000
view1	AlphaState	0
view1	ZTDStackMode	0
view1	ZTDStackValue	0
view	count	1

TIRF Microscope

Together with the Confocal Microscope, LAMAF has a LASER-TIRF equipment (Total Internal Reflection Fluorescence) mounted on a Nikon motorized inverted microscope. Read more…

2PHOTON

Two Photon Microscope

2PH microscopy is a cutting edge technique that allows the visualization of living tissues with a spatial resolution up to 1mm. This microscope is a specialized variant of the Confocal Microscope allowing for a lower phototoxicity. Read more…

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