Amaury Pupo

Postdoctoral Researcher
Centro Interdisciplinario de Neurociencia de Valparaíso
Laboratory of Molecular Sensors
Principal Investigator: Dr. Carlos González
Ph.D. in Sciences, mention Neuroscience, Universida de Valparaíso, Chile (2016).
Master degree in Biochemistry, mention Immunology, University of Havana, Cuba (2008)
Bachelor degree in Biochemistry, University of Havana, Cuba (2004)

Curriculum Vitae

Contact Information:
E-mail: amaury.pupo@gmail.com
Telephone: (56)-(32)-2508088
Fax: (56)-(32)-228-4440
Address: Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias,
Universidad de Valparaíso. Gran Bretaña 1111. Playa Ancha, Valparaíso-Chile.

Research Statement

Proteins are the ultimate responsible for biological function at the molecular level. I’ve always been fascinated by such bio-molecules, trying to understand how their function is determined by their structures. In few proteins the functional activity can be so directly and quantitatively measured as in ion channels. Ion channels constitute a family of membrane proteins present in all kingdom of life, playing vital and diverse roles.
Combining molecular biology and electrophysiological approaches with molecular and kinetic modeling, structural and computational biology, we are addressing unanswered issues regarding voltage-gated proton channel Hv1 gating and proton permeation, as well as connexin hemichannels regulation.

Publications

  • Fernandez A, Pupo A, Mena-Ulecia K, Gonzalez C. Pharmacological modulation of proton channel Hv1 in cancer therapy: Future perspectives. Mol Pharmacol. 2016 Jun 3. pii: mol.116.103804.
  • Pinto BI, García IE, Pupo A, Retamal MA, Martínez AD, Latorre R, González C. Charged residues at the first transmembrane region contribute to the voltage dependence of connexins slow gate. J Biol Chem. 2016 May 3. pii: jbc.M115.709402.
  • García IE, Prado P, Pupo A, Jara O, Rojas-Gómez D, Mujica P, Flores-Muñoz C, González-Casanova J, Soto-Riveros C, Pinto BI, Retamal MA, González C, Martínez AD. Connexinopathies: a structural and functional glimpse. BMC Cell Biol. 2016 May 24;17 Suppl 1:17. doi: 10.1186/s12860-016-0092-x.
  • García IE, Bosen F, Mujica P, Pupo A, Flores-Muñoz C, Jara O, Gonzalez C., Willecke K, Martinez AD. From hyperactive Connexin26 hemichannels to impairments in epidermal calcium gradient and permeability barrier in the Keratitis-Ichthyosis-Deafness syndrome. Journal of Investigative Dermatology. Jan 2016.
  • Retamal MA, Garcia IE, Pinto B, Pupo A, Baez-Nieto D, Stebherg J, Del Rio R, Gonzalez C. Extracellular Cysteine in Connexins: Role as Redox Sensors. Frontiers in Physiology 12/2015.
  • Morera FJ, Saravia J, Pontigo JP, Vargas-Chacoff L, Contreras GF, Pupo A, Lorenzo Y, Castillo K, Tilegenova C, Cuello LG, Gonzalez C. Voltage-dependent BK and Hv1 channels expressed in non-excitable tissues: New therapeutics opportunities as targets in human diseases. Pharmacol Res. 2015 Nov; 01:56-64. doi: 10.1016/j.phrs.2015.08.011. 
  • Castillo K, Pupo A, Baez-Nieto D, Contreras GF, Morera FJ, Neely A, Latorre R, Gonzalez C. Voltage-gated proton (Hv1) channels, a singular voltage sensing domain. FEBS Lett. 2015 Nov 14;589(22):3471-8. doi: 10.1016/j.febslet.2015.08.003.
  • Retamal MA, León-Paravic CG, Ezquer M, Ezquer F, Rio RD, Pupo A, Martínez AD, González C. Carbon monoxide: A new player in the redox regulation of connexin hemichannels. IUBMB Life. 2015 May 29. doi: 10.1002/iub.1388. 
  • Castillo K, Contreras GF, Pupo A, Torres YP, Neely A, González C, Latorre R. Molecular mechanism underlying β1 regulation in voltage- and calcium-activated potassium (BK) channels. Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4809-14. doi: 10.1073/pnas.
  • Pupo A, Gonzalez León C. In pursuit of an inhibitory drug for the proton channel. Proc Natl Acad Sci U S A. 2014 Jun 11. pii: 201408808. 
  • Tundidor Y, García-Hernández CP, Pupo A, Infante YC, Rojas G. Delineating the functional map of the interaction between nimotuzumab and the epidermal growth factor receptor. MAbs. 2014 Jul 1;6(4):1013-25. doi: 10.4161/mabs.28915. Epub 2014 Apr 23.
  • Pupo A, Baez-Nieto D, Martínez A, Latorre R, González C. Proton channel models: Filling the gap between experimental data and the structural rationale. Channels (Austin). 2014 Apr 22;8(3).
  • Infante YC, Pupo A, Rojas G. A combinatorial mutagenesis approach for functional epitope mapping on phage-displayed target antigen: application to antibodies against epidermal growth factor. MAbs. 2014 May-Jun;6(3):637-48. doi: 10.4161/mabs.28395.
  • Rojas G, Cabrera Infante Y, Pupo A, Carmenate T. Fine epitope specificity of antibodies against Interleukin-2 explains their paradoxical immunomodulatory effects. MAbs. 2014 Jan 1;6(1):273-85. doi: 10.4161/mabs.27224. Epub 2013 Nov 19.
  • Alvarez-Ginarte YM, Montero-Cabrera LA, García-de la Vega JM, Bencomo-Martínez A, Pupo A, Agramonte-Delgado A, Marrero-Ponce Y, Ruiz-García JA, Mikosch H. Integration of ligand and structure-based virtual screening for identification of leading anabolic steroids. J Steroid Biochem Mol Biol. 2013 Nov;138:348-58. doi: 10.1016/j.jsbmb.2013.07.004. Epub 2013 Jul 18.
  • Lamdan H, Gavilondo JV, Muñoz Y, Pupo A, Huerta V, Musacchio A, Pérez L, Ayala M, Rojas G, Balint RF, Larrick JW. Affinity maturation and fine functional mapping of an antibody fragment against a novel neutralizing epitope on human vascular endothelial growth factor. Mol Biosyst. 2013 Aug;9(8):2097-106. May 24.
  • Rojas G, Pupo A, Gómez S, Krengel U, Moreno E. Engineering the binding site of an antibody against N-glycolyl GM3: from functional mapping to novel anti-ganglioside specificities. ACS Chem Biol. 2013 Feb 15;8(2):376-86. doi: 10.1021/cb3003754. Epub 2012 Nov 21.
  • Rojas G, Pupo A, Leon K, Avellanet J, Carmenate T, Sidhu S. Deciphering the molecular bases of the biological effects of antibodies against Interleukin-2: a versatile platform for fine epitope mapping. Immunobiology. 2013 Jan;218(1):105-13. doi: 10.1016/j.imbio.2012.02.009. Epub 2012 Feb 16.
  • Bencomo-Martínez, A., Sablón-Carrazana, M., Rivera-Marrero, S., Rodríguez-Tanty, C., Álvarez-Ginarte, Y. M., Pupo-Meriño, A., Rivera-, S., et al. Identificación y caracterización in silico de la zona de interación entre el péptido beta-amiloide y compuestos derivados del naftaleno. Revista CENIC Ciencias Químicas, 2012, 43(1).
  • Talavera A, Friemann R, Gómez-Puerta S, Martinez-Fleites C, Garrido G, Rabasa A, López-Requena A, Pupo A, Johansen RF, Sánchez O, Krengel U, Moreno E. Nimotuzumab, an antitumor antibody that targets the epidermal growth factor receptor, blocks ligand binding while permitting the active receptor conformation. Cancer Res. 2009 Jul 15;69(14):5851-9. Epub 2009 Jul 7. Erratum in: Cancer Res. 2009 Aug 15;69(16):6758.
  • Pupo A, Moreno E. Do rotamer libraries reproduce the side-chain conformations of peptidic ligands from the PDB? J Mol Graph Model. 2009 Jan;27(5):611-9.
  • Rojas G, Pupo A, Del Rosario Aleman M, Vispo NS. Preferential selection of Cys-constrained peptides from a random phage-displayed library by anti-glucitollysine antibodies. J Pept Sci. 2008 Nov;14(11):1216-21.
  • Valiente PA, Batista PR, Pupo A, Pons T, Valencia A, Pascutti PG. Predicting functional residues in Plasmodium falciparum plasmepsins by combining sequence and structural analysis with molecular dynamics simulations. Proteins. 2008 Nov 1;73(2):440-57.
  • Pazos F, Valle A, Martínez D, Ramírez A, Calderón L, Pupo A, Tejuca M, Morera V, Campos J, Fando R, Dyszy F, Schreier S, Horjales E, Alvarez C, Lanio ME, Lissi E. Structural and functional characterization of a recombinant sticholysin I (rSt I) from the sea anemone Stichodactyla helianthus. Toxicon. 2006 Dec 15;48(8):1083-94.

 

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