Ana M. Cárdenas

Cell SignalingAna-maria-cardenas

Full Professor
Centro Interdisciplinario de Neurociencia de Valparaíso
Universidad de Valparaíso

Curriculum Vitae

Contact information:

E-mail: ana.cardenas at uv.cl
Phone: 56-32-2508052
Address: Gran Bretaña 1111, Playa Ancha. Valparaíso. Chile.

 

Research Statement:

Our group studies the molecular mechanisms that control vesicle trafficking and exocytosis in neurons, neuroendocrine cells and myocytes, with a special focused in pathogenic mechanisms involved in neuropathies, myopathies and Down syndrome. To this aim, we use techniques such as amperometry and total internal reflection fluorescence microscope (TIRFM) that enable us to monitor single exocytosis events in real time, in three different types of cells: (1) adrenal chromaffin cells, which release adrenaline, neuropeptides and other chemicals in response to stress, (2) cell lines derived from hippocampus and cerebral cortex of trisomy 16 mice, an animal model of Down syndrome, and (3) a human skeletal muscle cell line. 

 

Collaborations:

1995- : With Dr. P. Caviedes (I.C.B.M., Facultad de Medicina, Universidad de Chile): Intracellular calcium homeostasis in neuronal cell lines derived from trisomy 16 mouse, a model of human trisomy 21 (Dow Syndrome) Granted by DIPUV 2896 y 0398 and Fondecyt 1980906, 1040862, 1090160.

2009- : With Dr. Stéphane Gasman (CNRS, France): Molecular mechanisms of vesicle endocytosis in neuroendocrine cells. Granted by Programa de Colaboración Internacional ECOS/Conicyt.

2012- : With P. Caviedes and Jorge Bevilacqua  (I.C.B.M., Facultad de Medicina, Universidad de Chile): Molecular and cellular mechanisms of muscular dystrophy related to mutations of dysferlin. Proyecto Anillo ACT1121.

Selected Publications:

  • García-Palomero E, Montiel C, García AG, Alvarez R, Arnalich FM, Herrero C, Renart J, Lara H, Cárdenas AM (2000) “Multiple calcium pathways induce the expression of SNAP-25 protein in chromaffin cells” J. Neurochem. 74:1049-1058.
  • Montiel C, Mendoza I, Garcia CJ, Awad Y, Olivares-Garcia J, Solis-Garrido LM, Lara H, García AG, Cárdenas AM (2003) “Distinct Protein Kinases Regulate the SNAP-25 Expression in Chromaffin Cells”. Journal of Neuroscience Research 71, 353-364.
  • Mendoza IE, Schmachtenberg O, Tonk E, Fuentealba J, Díaz-RayaP, Lagos VL, García AG, Cárdenas AM (2003) Depolarization-induced ERK phosphorylation depends on the cytosolic Ca2+ level rather than on the Ca2+ channel subtype of chromaffin cells. J. Neurochem 86, 1477-1486.
  • Ardiles A, Maripillan J, Lagos V, Toro R, Mora IG, Villarroel L, Ales E, Borges R, Cárdenas AM (2006) A rapid exocytosis mode in chromaffin cells with a neuronal phenotype. J. Neurochem. 99:29-41.
  • Ardiles A, González-Jamett AM, Maripillan J, Naranjo D, Caviedes P, Cárdenas AM (2007) Calcium channel subtypes differentially regulate fusion pore stability and expansion. J. Neurochem. 103(4):1574-81.
  • Gonzalez-Jamett AM, Báez-Matus X, Hevia MA, Guerra MJ, Olivares MJ, Martínez AD, Neely A, Cárdenas AM (2010) The association of dynamin with synaptophysin regulates quantal size and duration of exocytotic events in chromaffin cells. J. Neurosci 30(32):10683-10691. 
  • González-Jamett AM, Haro-Acuña V, Momboisse F, Caviedes P, Bevilacqua JA, Cárdenas AM. (2013) Dynamin-2 in nervous system disorders. J Neurochem. Sep 18. doi: 10.1111/jnc.12455. [Epub ahead of print] 
  • Gonzalez-Jamett AM, Momboisse F, Guerra MJ, Ory S, Baez-Matus X, Barraza N, Calco V, Houy S, Couve E, Neely A, Martínez A, Gasman S, Cárdenas AM (2013) Dynamin-2 regulates fusion pore expansion and quantal release through a mechanism that involves actin dynamics in neuroendocrine chromaffin cells. PLOS ONE, 8(8):70638.  
  • González-Jamett AM, Momboisse F, Haro-Acuña V, Bevilacqua JA, Caviedes P, Cárdenas AM. (2013) Dynamin-2 Function and Dysfunction Along the Secretory Pathway. Front Endocrinol (Lausanne). 2013 Sep 18;4:126.